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Then, 250 L of 1.2 M PCA was added to stop the reaction and samples were centrifuged at 1600 g for 5 min

The pain report provides valuable insights for individuals with a history of physical trauma, as well as those experiencing musculoskeletal, neuropathic pain, fibromyalgia, and arthritis, including osteoarthritis and rheumatoid arthritis

10.1038/cr.2010.178 74 MorrisD.GuerraC.DonohueC.OhH.KhurasanyM.VenketaramanV

These include POLK, NIFK, SRGN, CAMP, CD109, and PLAC1 that are upregulated in several cancers resulting in metastasis and poor prognosis (Table 2, Fig

Western blotting Cell pellets were lysed in RIPA buffer (25 mM Tris-HCl, pH = 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with protease inhibitor cocktail (Sigma) and phosphatase Inhibitor Cocktail 2 and 3 (Sigma)